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Establishment and validation of a method for cell irradiation in 96-well and 6-well plates using a linear accelerator

【摘要】Toestablishandvalidateamethodforcellirradiationin96-welland6-wellplatesusingalinearaccelerator,threeirradiationmethods(G0B0F40,G0B1.5F40,andG180B1.5F40)weredesignedtoirradiatecellcultureplasticwaresimulatedwithRW3slabphantomandpolystyrene.Thedifferencebetweentheactualphysicalmeasureddoseandthepresetdosewascomparedamongthethreemethodsunderthepreparatoryconditionsof2,4,6,8,and10Gy.MDA-MB-231cellswereanalyzedbyusingacellproliferationassayandaclonogenicassaytoverifythedifferencebetweenthethreecellirradiationmethodsoncellradiosensitivity.Foreachpresetdose,thedifferencebetweentheactualmeasureddoseandthepresetdosewasthelowestforMethodG0B1.5F40,thesecondlowestforMethodG180B1.5F40,andthemaximumforMethodG0B0F40.Therangesofthedifferenceswere-0.28to0.02%,-2.17to-1.80%,and-4.92to-4.55%,and0.31to-0.12%,-3.42to-2.86%,and-7.31to-6.92%,respectively,for96-welland6-wellplates.ThecellcultureexperimentsprovedthatMethodG0B1.5F40wasanaccurate,effective,simple,andpracticalirradiationmethod.Themostaccurateandeffectivecellirradiationmethodshouldalwaysbeused,asitwillreducedosedifferencesandinstabilityfactorsandprovideimprovedaccuracyandcomparabilityforlaboratoriesresearchingcellularradiosensitivity.

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199 共8页 核技术:英文版 2018年5期 免费 中文科技期刊数据库

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